New immunochemical method for determination of bromopropylate in wine

Profil Titel	New immunochemical method for determination of bromopropylate in wine
Referenz	11 ES 28G2 3LF7
Herkunftsland	Spain
Kooperationsart	Angebot
Eintrag/Änderung	2011-05-26 / 2011-05-26
Status	Dieses Profil ist abgelaufen! Falls Sie denoch Interesse haben, kontaktieren Sie uns. (2012-05-19)

Kurzfassung

A Spanish research organisation has developed a technique for the detection and quantification of bromopropylate using an inmunochemical method. This method is rapid and efficient, able to analyze the presence of this miticide, down to 0.14 microg/L, directly in wine samples or other foods of plant origin without extraction or pre-concentration steps. Companies interested in a licence agreement and/or in collaboration on the development of this technology are sought.

Details

Bromopropylate (BP) is a miticide extensively used in agriculture against all stages of mites. Its use is banned in some European countries but it is allowed in others, waiting for environmental better alternatives, establishing the maximum residue levels in wine grapes or citrus fruits in 2 mg/kg (Commission regulation (EC) nº149/2008).

The first immunochemical method that allows direct determination of bromopropylate in wine, fruits or other foods of plant origin has been developed. This method based on an indirect competitive ELISA, can detect BP down to 0.14 microg/L, a detection limit far higher than that of the current methods, based in chromatographic techniques, which is around the MRL.

Presence of bromopropylate and other related pesticides may be quantified using this technique without the interference of other pesticides.

Innovative Aspects:
The main features of the developed technique are:

• Specificity. Cross-reactivity with other pesticides, such as DDT or atrazine, is negligible, except to those with a bis-halophenyl group in their structure.

• High sensitivity. Limit of detection of BP down to 0.16 microg/L, far below the MRL established in Europe.

• The system is rapid, easy to use (less than 75 min). BP can be directly analyzed in white wine samples without any previous extraction or pre-concentration, which is required in the current analytical methods (GC and HPLC).

• It allows simultaneous analysis of multiple samples (HTS).